dpp-4 inhibitor screening test assay kit  (Cayman Chemical)

Journal: American Journal of Translational Research

Article Title: Sitagliptin protects against hypoxia/reoxygenation (H/R)-induced cardiac microvascular endothelial cell injury

doi:

Figure Lengend Snippet: Hypoxia/reoxygenation (H/R) increased the expression of DPP-4 in primary cardiac microvascular endothelial cells (CMECs). Hypoxia/reoxygenation experiments were performed by putting cells in an air-tight chamber with pure N2 followed by incubation at 37°C for 6 h. Cells were then cultured with normal medium in a normoxic incubator (95% air plus 5% CO2) for 12 h for reoxygenation. A. Expression of DPP-4 at the mRNA levels was determined by real time PCR analysis; B. Expression of DPP-4 at the protein levels was determined by western blot analysis (*, P<0.01 vs. the control group, n=6).

Article Snippet: The following antibodies were used in this study: DPP-4 (1:1000, #ab28340, Abcam, USA); NOX-4 (1:2000, ab133303, Abcam, USA); p-p38 (1:1000, #9216, Cell Signaling Technology, USA); p38 (1:3000, #8690, Cell Signaling Technology, USA); p65 (1:2000, #9460, Cell signaling technology, USA); Lamin B1 (1:5000, #13435, Cell Signaling Technology, USA); β-actin (1:10000, #3700, Cell Signaling Technology, USA); anti-rabbit IgG, HRP-linked antibody (1:2000, #7074, Cell Signaling Technology, USA); anti-mouse IgG, HRP-linked antibody (1:2000, #7076, Cell Signaling Technology, USA).

Techniques: Expressing, Incubation, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot